Presentation

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The research focus of the Translational Proteomics Research Lab is the application of mass spectrometry (MS) based proteomics to understand human health and disease. The study of the proteome of patients, integrated with their clinical data, could lead to the improvement of patient diagnosis and stratification, contributing to development and implementation of precision medicine.

In the heart of one of the major research hospitals in France, the Translational Proteomics Research Lab develops innovative translational proteomics approaches, aiming to apply them to clinical research and transfer them into clinical practices.

The dynamic interaction between the Translational Proteomics Research Lab and the Necker Proteomics Platform ensures that cutting-edge mass spectrometry technology is available to all research teams.

Location

 

Plateforme Protéomique PPN Necker

Faculté de Médecine Université de Paris

156-160 rue de Vaugirard

75015 Paris

 

Tél : 01 40 61 54 67

proteomique.sfrnecker@inserm.fr

 

PPN Necker is at 1st flour of the Faculty building

Staff

 
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Chiara Guerrera, PhD

Scientific director - IR INSERM

chiara.guerrera@inserm.fr

Cerina Chhuon

AI-Universite de Paris

cerina.chhuon@inserm.fr

Joanna Lipecka
Vincent Jung
Kevin Roger
Sara Ceccacci
 

Equipement

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NEW 2021!!! 

 

TimsTOF Pro MS (Bruker)

 

coupled to EVOSEPOne 

nanochromatographer

Current Areas of Research

1. Precision proteomics for specific diseases. We are currently working on innovative proteomics approaches to :

 

   a. Find biomarkers for treatment response in Cystic fibrosis patients analyzing the sweat proteome;

   b. Understand mechanisms of myelomeningocele to improve prenatal cellular therapy through analysis of amniotic fluid;

   c. Understand Focal Epilepsy by studying LCR and brain biopsies to better stratify patients and personalize their treatment;

   d. Find biomarkers for treatment response and prognosis in Cystinuria patients by studying the proteome of urine and exosomes therein.

 

2. Universal analysis of plasma proteome to establish a personalized quantitative profiling (BloodID). BloodID profiling will integrate soluble plasmatic proteins and the circulating extracellular vesicles carrying cellular information. We believe that BloodID will allow the monitoring of individuals’ health and, in time, be transferrable in clinical practice.

 

Publications 2015-2021

 

For the full list of  publications click here 

 

*** publication en 1er ou dernier auteur par les ingénieurs de la plateforme

 

  1. The pentose phosphate pathway constitutes a major metabolic hub in pathogenic Francisella. Rytter H, Jamet A, Ziveri J, Ramond E, Coureuil M, Lagouge-Roussey P, Euphrasie D, Tros F, Goudin N, Chhuon C, Nemazanyy I, de Moraes FE, Labate C, Guerrera IC, Charbit A. PLoS Pathog. 2021 Aug 2;17(8):e1009326.

  2. RESVEGA, a Nutraceutical Omega-3/Resveratrol Supplementation, Reduces Angiogenesis in a Preclinical Mouse Model of Choroidal Neovascularization. Courtaut F, Aires V, Acar N, Bretillon L, Guerrera IC, Chhuon C, Pais de Barros JP, Olmiere C, Delmas D. Int J Mol Sci. 2021 Oct 13;22(20):11023.

  3. STING protects breast cancer cells from intrinsic and genotoxic-induced DNA instability via a non-canonical, cell-autonomous pathway. Cheradame L, Guerrera IC, Gaston J, Schmitt A, Jung V, Goudin N, Pouillard M, Radosevic-Robin N, Modesti M, Judde JG, Cairo S, Goffin V. Oncogene. 2021 Oct 8.

  4. Polo-like kinase 1 (Plk1) regulates DNA replication origin firing and interacts with Rif1 in Xenopus. Ciardo D, Haccard O, Narassimprakash H, Cornu D, Guerrera IC, Goldar A, Marheineke K. Nucleic Acids Res. 2021 Sep 27;49(17):9851-9869.

  5. Synaptic disruption and CREB-regulated transcription are restored by K+ channel blockers in ALS. Catanese A, Rajkumar S, Sommer D, Freisem D, Wirth A, Aly A, Massa-López D, OlivieriA, Torelli F, Ioannidis V, Lipecka J, Guerrera IC, Zytnicki D, Ludolph A, Kabashi E, Mulaw MA, Roselli F, Böckers TM. EMBO Mol Med. 2021 Jul 7;13(7):e13131.

  6. Identification and Relative Quantification of hFSH Glycoforms in Women's Sera via MS-PRM-Based Approach. Melchiorre C, Chhuon C, Jung V, Lipecka J, Di Rella F, Conforti A, Amoresano A, Carpentieri A, Guerrera IC. Pharmaceutics. 2021 May 27;13(6):798.

  7. Glucose treatment of human pancreatic β-cells enhances translation of mRNAs involved in energetics and insulin secretion. Zakaria A, Berthault C, Cosson B, Jung V, Guerrera IC, Rachdi L, Scharfmann R. J Biol Chem. 2021 Jul;297(1):100839.

  8. Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1. González-Barriga A, Lallemant L, Dincã DM, Braz SO, Polvèche H, Magneron P, Pionneau C, Huguet-Lachon A, Claude JB, Chhuon C, Guerrera IC, Bourgeois CF, Auboeuf D, Gourdon G, Gomes-Pereira M. Front Cell Neurosci. 2021 May 5;15:662035.

  9. Role of VAMP7-dependent secretion of reticulon 3 in neurite growth. Wojnacki J, Nola S, Bun P, Cholley B, Filippini F, Pressé MT, Lipecka J, Lam SM, N'guyen J, Simon A, Ouslimani A, Shui G, Fader CM, Colombo MI, Guerrera IC, Galli T. Cell Rep. 2021 Apr 13;35(2):109006.

  10. Sickle Cell Trait Modulates the Proteome and Phosphoproteome of Plasmodium falciparum-Infected Erythrocytes.Chauvet M, Chhuon C, Lipecka J, Dechavanne S, Dechavanne C, Lohezic M, Ortalli M, Pineau D, Ribeil JA, Manceau S, Le Van Kim C, Luty AJF, Migot-Nabias F, Azouzi S, Guerrera IC, Merckx A. Front Cell Infect Microbiol. 2021 Mar 24;11:637604.

  11. The equilibrative nucleoside transporter 1 (ENT1) is critical for nucleotide homeostasis and optimal erythropoiesis. Mikdar M, Gonzalez-Menendez P, Cai X, Zhang Y, Serra M, Dembélé AK, Boschat AC, Sanquer S, Chhuon C, Guerrera IC, Sitbon M, Hermine O, Colin Y, Le Van Kim C, Kinet S, Narla M, Xia Y, Peyrard T, Taylor N, Azouzi S. Blood. 2021 Mar 10:blood.2020007281. doi: 10.1182/blood.2020007281. Epub ahead of print. PMID: 33690842.

  12. Disruption of pathways regulated by Integrator complex in Galloway-Mowat syndrome due to WDR73 mutations. Tilley FC, Arrondel C, Chhuon C, Boisson M, Cagnard N, Parisot M, Menara G, Lefort N, Guerrera IC, Bole-Feysot C, Benmerah A, Antignac C, Mollet G. Sci Rep. 2021 Mar 8;11(1):5388. doi: 10.1038/s41598-021-84472-7. PMID: 33686175; PMCID: PMC7940485.

  13. Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth. Wojnacki J, Nola S, Bun P, Cholley B, Filippini F, Pressé MT, Lipecka J, Man Lam S, N'guyen J, Simon A, Ouslimani A, Shui G, Fader CM, Colombo MI, Guerrera IC, Galli T. Cell Rep. 2020 Dec 22;33(12):108536. doi: 10.1016/j.celrep.2020.108536. PMID: 33357422.

  14. GFOGER Peptide Modifies the Protein Content of Extracellular Vesicles and Inhibits Vascular Calcification. Mansour A, Darwiche W, Yaker L, Da Nascimento S, Gomila C, Rossi C, Jung V, Sonnet P, Kamel S, Guerrera IC, Boullier A, Ausseil JFront Cell Dev Biol. 2020 Nov 30;8:589761. doi: 10.3389/fcell.2020.589761. PMID: 33330469; PMCID: PMC7734313.

  15. Dataset of a comparative proteomics experiment in a methylmalonyl-CoA mutase knockout HEK 293 cell model. Costanzo M, Caterino M, Cevenini A, Jung V, Chhuon C, Lipecka J, Fedele R, Guerrera IC, Ruoppolo M. Data Brief. 2020 Oct 22;33:106453. doi: 10.1016/j.dib.2020.106453. PMID: 33195772; PMCID: PMC7644733.

  16. Urinary Exosomes of Patients with Cystic Fibrosis Unravel CFTR-Related Renal Disease. Gauthier S, Pranke I, Jung V, Martignetti L, Stoven V, Nguyen-Khoa T, Semeraro M, Hinzpeter A, Edelman A, Guerrera IC, Sermet-Gaudelus I. Int J Mol Sci. 2020 Sep 10;21(18):6625. doi: 10.3390/ijms21186625. PMID: 32927759; PMCID: PMC7554933.

  17. *** A sensitive S-Trap-based approach to the analysis of T cell lipid raft proteome. Chhuon C, Zhang SY, Jung V, Lewandowski D, Lipecka J, Pawlak A, Sahali D, Ollero M, Guerrera IC. J Lipid Res. 2020 Nov;61(11):1512-1523. doi: 10.1194/jlr.D120000672. Epub 2020 Aug 7. PMID: 32769147; PMCID: PMC7604723.

  18. Proteomics Reveals that Methylmalonyl-CoA Mutase Modulates Cell Architecture and Increases Susceptibility to Stress. Costanzo M, Caterino M, Cevenini A, Jung V, Chhuon C, Lipecka J, Fedele R, Guerrera IC, Ruoppolo M. Int J Mol Sci. 2020 Jul 15;21(14):4998. doi: 10.3390/ijms21144998. PMID: 32679819; PMCID: PMC7403994.

  19. Critical Role of a Sheath Phosphorylation Site On the Assembly and Function of an Atypical Type VI Secretion System. Ziveri J, Chhuon C, Jamet A, Rytter H, Prigent G, Tros F, Barel M, Coureuil M, Lays C, Henry T, Keep NH, Guerrera IC, Charbit A. Mol Cell Proteomics. 2019 Dec;18(12):2418-2432. doi: 10.1074/mcp.RA119.001532. Epub 2019 Oct 2. PMID: 31578219; PMCID: PMC6885697.

  20. STK38 kinase acts as XPO1 gatekeeper regulating the nuclear export of autophagy proteins and other cargoes.  Martin AP, Jacquemyn M, Lipecka J, Chhuon C, Aushev VN, Meunier B, Singh MK, Carpi N, Piel M, Codogno P, Hergovich A, Parrini MC, Zalcman G, Guerrera IC, Daelemans D, Camonis JH. EMBO Rep. 2019 Nov 5;20(11):e48150. doi: 10.15252/embr.201948150.

  21. Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome.  Arrondel, Missoury, Snoek, Patat, Menara, Collinet, Liger, Durand, Gribouval, Boyer, Buscara, Martin, Machuca, Nevo, Lescop, Braun, Boschat, Sanquer, Guerrera IC, Revy, Parisot, Masson, BODDAERT, Charbit, Decramer, Novo, Macher, Ranchin, Bacchetta, Laurent, Collardeau-Frachon, Albertien van Eerde, Hildebrandt, Magen, Antignac, van Tilbeurgh, and Mollet.  Nat Commun. 2019 Sep 3;10(1):3967. doi: 10.1038/s41467-019-11951-x

  22. Intracellular offsprings released from SFB filaments are flagellated. Nkamba I, Mulet C, Dubey GP, Gorgette O, Couesnon A, Salles A, Moya-Nilges M, Jung V, Gaboriau-Routhiau V, Guerrera IC, Shima T, Umesaki Y, Nigro G, Krijnse-Locker J, Berard M, Cerf-Bensussan1 N, Sansonetti PJ, Schnupf P., Nat Microbiol. 2020 Jan;5(1):34-39. doi: 10.1038/s41564-019-0608-1.

  23. CMIP is a negative regulator of T cell signaling. Oniszczuk J, Sendeyo K, Chhuon C, Savas B, Cogné E, Vachin P, Henique C, Guerrera IC, Astarita G, Frontera V, Pawlak A, Audard V, Sahali D, Ollero M. Cell Mol Immunol. 2019 Aug 8. doi: 10.1038/s41423-019-0266-5. PubMed PMID: 31395948.

  24. Essential role of GEXP15, a specific Protein Phosphatase type 1 partner, in Plasmodium berghei in asexual erythrocytic proliferation and transmission. Hollin T, De Witte C, Fréville A, Guerrera IC, Chhuon C, Saliou JM, Herbert F, Pierrot C, Khalife J. PLoS Pathogens 2019 Jul 26;15(7):e1007973. doi:10.1371/journal.ppat.1007973. PMID: 31348803

  25. Plasmodium pseudo-Tyrosine Kinase-like binds PP1 and SERA5 and is exported to host erythrocytes Gnangnon B, Fréville A, Cailliau K, Leroy C, De Witte C, Tulasne D, Martoriarti A, Jung V, Guerrera IC, Marion S, Khalife J, Pierrot C. Sci Rep. 2019 May 31;9(1):8120. doi: 10.1038/s41598-019-44542-3. PubMed PMID: 31148576;

  26. Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion. Dupont MA, Humbert C, Huber C, Siour Q, Guerrera IC, Jung V, Christensen A, Pouliet A, Garfa-Traore M, Nitschké P, Injeyan M, Millar K, Chitayat D, Shannon P, Girisha KM, Shukla A, Mechler C, Lorentzen E, Benmerah A, Cormier-Daire V, Jeanpierre C, Saunier S, Delous M. Hum Mol Genet. 2019. pii: ddz091. doi: 10.1093/hmg/ddz091.

  27. Interaction between galectin-3 and cystinosin uncovers a pathogenic role of inflammation in kidney involvement of cystinosis. Lobry T, Miller R, Nevo N, Rocca CJ, Zhang J, Catz SD, Moore F, Thomas L, Pouly D, Bailleux A, Guerrera IC, Gubler MC, Cheung WW, Mak RH, Montier T, Antignac C, Cherqui S. Kidney Int. 2019 Mar 6. pii: S0085-2538(19)30172-3. doi: 10.1016/j.kint.2019.01.029.

  28. Chronic Staphylococcus aureus lung infection correlates with proteogenomic and metabolic adaptations leading to an increased intracellular persistence. Tan X, Coureuil M, Ramond E, Euphrasie D, Dupuis M, Tros F, Meyer J, Nemazanyy I, Chhuon C, Guerrera IC, Ferroni A, Sermet-Gaudelus I, Nassif X, Charbit A, Jamet A. Clin Infect Dis. 2019 Feb 7. doi: 10.1093/cid/ciz106.

  29. LC-MS/MS-based quantification of efflux transporter proteins at the BBB. Gomez-Zepeda D, Taghi M, Smirnova M, Sergent P, Liu WQ, Chhuon C, Vidal M, Picard M, Thioulouse E, Broutin I, Guerrera IC, Scherrmann JM, Parmentier Y, Decleves X, Menet MC. J Pharm Biomed Anal. 2019 Feb 5;164:496-508. doi: 10.1016/j.jpba.2018.11.013. Epub 2018 Nov 8.

  30. ***High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect. Medina-Cano D, Ucuncu E, Nguyen LS, Nicouleau M, Lipecka J, Bizot JC, Thiel C, Foulquier F, Lefort N, Faivre-Sarrailh C, Colleaux L, Guerrera IC, Cantagrel V. Elife. 2018 Oct 12;7. pii: e38309. doi: 10.7554/eLife.38309.

  31. ***Comparative proteomics of respiratory exosomes in cystic fibrosis, primary ciliary dyskinesia and asthma. Rollet-Cohen V, Bourderioux M, Lipecka J, Chhuon C, Jung V, Mesbahi M, Nguyen-Khoa T, Guérin-Pfyffer S, Schmitt A, Edelman A, Sermet-Gaudelus I, Guerrera IC. J Proteomics. 2018 Aug 15;185:1-7. doi: 10.1016/j.jprot.2018.07.001. Epub 2018 Jul 3.PMID: 30032860

  32. M’baya-Moutoulaa, E, Louveta, L, Moliniéb R, Guerrera IC, Cerutti C, Fourdinier O, Nourrye V, Gutierrez L, Morlière P, Mesnard F, Ziad A.M., Metzinger-le Meutha V. , and Metzinger L (2018). A multi-omics analysis of the regulatory changes induced by miR-223 in a monocyte/macrophage cell line. Biochim Biophys Acta Mol Basis Dis. 2018 Aug;1864(8):2664-2678. doi: 10.1016/j.bbadis.2018.05.010.

  33. ***Rollet-Cohen, V., Bourderioux, M., Lipecka, J; Chhuon, C., Mesbahi M, Nguyen-Khoa, T; Guérin-Pfyffer, S.; Schmitt, A; Edelman, A; Sermet-Gaudelus, I, Guerrera, IC. (2018) Comparative proteomics of respiratory exosomes in Cystic Fibrosis, Primary Ciliary Dyskinesia and Asthma. J Proteomics. 2018 Aug 15;185:1-7. doi: 10.1016/j.jprot.2018.07.001

  34. Braun, D. A., Rao, J., Mollet, G., Schapiro, D., Daugeron, M.-C., Tan, W., Gribouval, O., Boyer, O., Revy, P., Jobst-Schwan, T., Schmidt, J. M., Lawson, J. A., Schanze, D., Ashraf, S., Ullmann, J. F. P., Hoogstraten, C. A., Boddaert, N., Collinet, B., Martin, G., Liger, D., Lovric, S., Furlano, M., Guerrera, I. C., Sanchez-Ferras, O., Hu, J. F., Boschat, A.-C., Sanquer, S., Menten, B., Vergult, S., De Rocker, N., Airik, M., Hermle, T., Shril, S., Widmeier, E., Gee, H. Y., Choi, W.-I., Sadowski, C. E., Pabst, W. L., Warejko, J. K., Daga, A., Basta, T., Matejas, V., Scharmann, K., Kienast, S. D., Behnam, B., Beeson, B., Begtrup, A., Bruce, M., Ch’ng, G.-S., Lin, S.-P., Chang, J.-H., Chen, C.-H., Cho, M. T., Gaffney, P. M., Gipson, P. E., Hsu, C.-H., Kari, J. A., Ke, Y.-Y., Kiraly-Borri, C., Lai, W.-M., Lemyre, E., Littlejohn, R. O., Masri, A., Moghtaderi, M., Nakamura, K., Ozaltin, F., Praet, M., Prasad, C., Prytula, A., Roeder, E. R., Rump, P., Schnur, R. E., Shiihara, T., Sinha, M. D., Soliman, N. A., Soulami, K., Sweetser, D. A., Tsai, W.-H., Tsai, J.-D., Topaloglu, R., Vester, U., Viskochil, D. H., Vatanavicharn, N., Waxler, J. L., Wierenga, K. J., Wolf, M. T. F., Wong, S.-N., Leidel, S. A., Truglio, G., Dedon, P. C., Poduri, A., Mane, S., Lifton, R. P., Bouchard, M., Kannu, P., Chitayat, D., Magen, D., Callewaert, B., van Tilbeurgh, H., Zenker, M., Antignac, C., and Hildebrandt, F. (2017) Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly. Nat. Genet. 49, 1529–1538

  35. Duriez, M., Mandouri, Y., Lekbaby, B., Wang, H., Schnuriger, A., Redelsperger, F., Guerrera, C. I., Lefevre, M., Fauveau, V., Ahodantin, J., Quetier, I., Chhuon, C., Gourari, S., Boissonnas, A., Gill, U., Kennedy, P., Debzi, N., Sitterlin, D., Maini, M. K., Kremsdorf, D., and Soussan, P. (2017) Alternative splicing of hepatitis B virus: A novel virus/host interaction altering liver immunity. J. Hepatol. 67, 687–699

  36. Gomez-Zepeda, D., Chaves, C., Taghi, M., Sergent, P., Liu, W.-Q., Chhuon, C., Vidal, M., Picard, M., Thioulouse, E., Broutin, I., Guerrera, I.C., Scherrmann, J.-M., Parmentier, Y., Decleves, X., and Menet, M.-C. (2017) Targeted unlabeled multiple reaction monitoring analysis of cell markers for the study of sample heterogeneity in isolated rat brain cortical microvessels. J. Neurochem. 142, 597–609

  37. Sicot, G., Servais, L., Dinca, D. M., Leroy, A., Prigogine, C., Medja, F., Braz, S. O., Huguet-Lachon, A., Chhuon, C., Nicole, A., Gueriba, N., Oliveira, R., Dan, B., Furling, D., Swanson, M. S., Guerrera, I. C., Cheron, G., Gourdon, G., and Gomes-Pereira, M. (2017) Downregulation of the Glial GLT1 Glutamate Transporter and Purkinje Cell Dysfunction in a Mouse Model of Myotonic Dystrophy. Cell Rep 19, 2718–2729

  38. ***Nevo, N., Thomas, L., Chhuon, C., Andrzejewska, Z., Lipecka, J., Guillonneau, F., Bailleux, A., Edelman, A., Antignac, C., and Guerrera, I. C. (2017) Impact of Cystinosin Glycosylation on Protein Stability by Differential Dynamic Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC). Mol. Cell Proteomics 16, 457–468

  39. Ziveri, J., Tros, F., Guerrera, I. C., Chhuon, C., Audry, M., Dupuis, M., Barel, M., Korniotis, S., Fillatreau, S., Gales, L., Cahoreau, E., and Charbit, A. (2017) The metabolic enzyme fructose-1,6-bisphosphate aldolase acts as a transcriptional regulator in pathogenic Francisella. Nat Commun 8, 85

  40. Meijer, B. M., Jang, S. M., Guerrera, I. C., Chhuon, C., Lipecka, J., Reisacher, C., Baleux, F., Sansonetti, P. J., Muchardt, C., and Arbibe, L. (2017) Threonine eliminylation by bacterial phosphothreonine lyases rapidly causes cross-linking of mitogen-activated protein kinase (MAPK) in live cells. J. Biol. Chem. 292, 7784–7794

  41. ***Chhuon, C., Pranke, I., Borot, F., Tondelier, D., Lipecka, J., Fritsch, J., Chanson, M., Edelman, A., Ollero, M., and Guerrera, I. C. (2016) Dataset of differential lipid raft and global proteomes of SILAC-labeled cystic fibrosis cells upon TNF -α stimulation. Data Brief 9, 51–56

  42. ***Chhuon, C., Pranke, I., Borot, F., Tondelier, D., Lipecka, J., Fritsch, J., Chanson, M., Edelman, A., Ollero, M., and Guerrera, I. C. (2016) Changes in lipid raft proteome upon TNF-α stimulation of cystic fibrosis cells. J Proteomics 145, 246–253

  43. ***Lipecka, J., Chhuon, C., Bourderioux, M., Bessard, M.-A., van Endert, P., Edelman, A., and Guerrera, I. C. (2016) Sensitivity of mass spectrometry analysis depends on the shape of the filtration unit used for filter aided sample preparation (FASP). Proteomics 16, 1852–1857

  44. ***Andrzejewska, Z., Nevo, N., Thomas, L., Chhuon, C., Bailleux, A., Chauvet, V., Courtoy, P. J., Chol, M., Guerrera, I. C., and Antignac, C. (2015) Cystinosin is a Component of the Vacuolar H+-ATPase-Ragulator-Rag Complex Controlling Mammalian Target of Rapamycin Complex 1 Signaling. J. Am. Soc. Nephrol.,

  45. Aoufouchi, S., De Smet, A., Delbos, F., Gelot, C., Guerrera, I. C., Weill, J.-C., and Reynaud, C.-A. (2015) 129-Derived Mouse Strains Express an Unstable but Catalytically Active DNA Polymerase Iota Variant. Mol. Cell. Biol. 35, 3059–3070

  46. Ramond, E., Gesbert, G., Guerrera, I. C., Chhuon, C., Dupuis, M., Rigard, M., Henry, T., Barel, M., and Charbit, A. (2015) Importance of host cell arginine uptake in Francisella phagosomal escape and ribosomal protein amounts. Mol. Cell Proteomics 14, 870–881