Presentation
Fluorescence microscopy plays an essential role in biomedical research, offering perspectives for exploring cellular and tissue mechanisms. Within the SFR Necker, our cellular imaging platform stands out for its sharp expertise in visualizing and analysing complex structures as well as dynamic processes. Our field of investigation extends from the microscopic scale, at the cellular level, to that of the entire organism, allowing for a thorough understanding of biological phenomena.
Our primary objective is to provide researchers with privileged access to state-of-the-art optical equipment, enabling them to successfully address the most complex scientific challenges. In parallel, our team actively engages in the development and implementation of innovative imaging methods to meet the evolving needs of the scientific community.
Open to all research institutes as well as private industries, our platform serves as a privileged exchange and collaboration hub conducive to the emergence of new ideas and the realization of ambitious projects in the fields of biology and medicine.
Localisation
The platform is located at the Imagine Institute and has a branch at the Necker Faculty of Medicine.
Cell Imaging Platform of the SFR Necker (INSERM US 24)
Imagine Institute, 24 bd du Montparnasse, 75015 Paris
Tel: 01 42 75 43 91
E-mail: imagerie.sfr.necker@inserm.fr
Personnel
Huyen Augis-Chu, PhD
IECN (CDD)
Nolwenn Brégent
IECN (CDD)
Equipment
The platform has a diverse range of microscopes that cover a wide range of techniques and enable observations at different scales.
For structural and live imaging:
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4 laser scanning confocal microscopes: 1 Nikon AX, 2 Leica SP8, 1 Leica SP5
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2 spinning disk confocal microscopes: Yokogawa CSU-X1 on Zeiss Axio-observer Z1
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4 wide-field microscopes: 2 Zeiss ApoTome structured illumination microscopes, 1 Nikon TIRF fluorescence microscope, 1 Zeiss Axiovert A1 inverted microscope
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1 high content screening confocal microscope : Opera Phenix (HCS)
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1 fully automated imaging system: Thermo Fisher EVOS M7000
For ultrastructure analysis:
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1 STED super-resolution microscope: Leica SP8-gSTED
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1 STORM super-resolution microscope: "home-made" on Nikon Eclipse Ti
For three-dimensional imaging:
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1 light sheet microscope: Zeiss Lightsheet Z1
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1 micro-computed tomography (microCT): Bruker Skyscan 1272
Other equipment:
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1 fluorescence stereomicroscope: Leica M205 FCA
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1 magnifier: Zeiss Stemi 305
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1 refractometer Mettler Toledo
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1 Ibidi Pump System
Missions
The main missions of the platform are to:
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advise users in the design and implementation of their experiments,
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assist users in sample preparation for super-resolution microscopy, expansion microscopy, or tissue clearing,
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provide users with imaging systems best suited to their scientific needs,
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assist and train users on all acquisition systems,
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collaborate with researchers on the use of advanced techniques,
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assist users in interpreting and formatting their results.
Fees
On request to meriem.garfa@inserm.fr
Image Gallery
TO COME
Jeanne Porée
Stagiaire M2 - Master Neurosciences
Béatrice Durel
IR INSERM
Publications
2023
The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells. Nature Communications, 2023 December
A.Claude-Taupin, P.Isnard, A.Bagattin, N.Kuperwasser, F.Roccio, B.Ruscica, N.Goudin, M.Garfa-Traoré, ..., F.Terzi, P.Codogno & N. Dupont
Fluid shear stress triggers cholesterol biosynthesis and uptake in inner medullary collecting duct cells, independently of nephrocystin-1 and nephrocystin-4. Frontiers in Molecular Biosciences, 2023 October
M.Garfa Traoré, F.Roccio, C.Miceli, G.Ferri, M.Parisot, N.Cagnard, M.Lhomme, N.Dupont, A.Benmerah, S.Saunier, M.Delous
DOCK11 deficiency in patients with X-linked actinopathy and autoimmunity. Blood, 2023 June
Boussard C, Delage L, Gajardo T, Kauskot A, Batignes M, Goudin N, Stolzenberg MC, Brunaud C, Panikulam P, Riller Q, Moya-Nilges M, Solarz J, Repérant C, Durel B, ... Ménager M, Sepulveda FE, Adam F, Rieux-Laucat F.
Bi-allelic variations in CRB2, encoding the crumbs cell polarity complex component 2, lead to non-communicating hydrocephalus due to atresia of the aqueduct of sylvius and central canal of the medulla. Acta Neuropathologica Communications, 2023 February
A.Tessier, N.Roux, L.Boutaud, E.Lunel, L.Hakkakian, M.Parisot, M.Garfa‐Traoré, ..., T.Attié‐Bitach and S.Thomas
Evaluation of Slowfade Diamond as a buffer for STORM microscopy. Biomed Opt Express, 2023 January.
Boukhatem H, Durel B, Raimbault M, Laurent A, Olivier N.
2022
UNC45A deficiency causes microvillus inclusion disease-like phenotype by impairing myosin VB-dependent apical trafficking. JCI, 2022 May.
R.Duclaux-Loras, C.Lebreton, J.Berthelet, F.Charbit-Henrion, O.Nicolle, C.Revenu des Courtils, S.Waich, T.Valovka, A.Khiat, M.Rabant, C.Racine, IC.Guerrera, J.Baptista, M.M Mahe, M.W Hess, B.Durel.... N.Cerf-Bensussan, M.Parlato.
Agonists of prostaglandin E2 receptors as potential first in class treatment for nephronophthisis and related ciliopathies. PNAS, 2022 May
H.Garcia, A.Serafin, F.Silbermann, E.Porée, A.Viau, C.Mahaut, K.Billot, E.Birgy, M.Garfa-Traore, .... M.Delous, L.Briseño-Roa, S.Saunier.
2021
Quantitative dSTORM super-resolution microscopy localizes Aurora kinase A/AURKA in the mitochondrial matrix. Biol Cell, 2021 November
B.Durel, C.Kervrann, G.Bertolin.
ATG9A protects the plasma membrane from programmed and incidental permeabilization. Nat.Cell Biol, 2021 August.
Claude-Taupin A, Jia J, Bhujabal Z, Garfa-Traoré M, ..., Reggiori F, Deretic V.
Three-dimensional architecture of nephrons in the normal and cystic kidney. Kidney Int., 2021 March
Blanc T, Goudin N, Zaidan M, Traore MG, Bienaime F, Turinsky L, Garbay S, Nguyen C, Burtin M, Friedlander G, Terzi F, Pontoglio M.
Mobilized Multipotent Hematopoietic Progenitors Promote Expansion and Survival of Allogeneic Tregs and Protect Against Graft Versus Host Disease. Front Immunol, 2021 February.
D'Aveni M, Notarantonio AB, Agbogan VA, Bertrand A, Fouquet G, Gastineau P, Garfa-Traoré M, De Carvalho M, Hermine O, Rubio MT, Zavala F.