CELL IMAGING
PRESENTATION
Fluorescence microscopy plays an essential role in biomedical research, offering perspectives for exploring cellular and tissue mechanisms. Within the SFR Necker,
our cellular imaging platform stands out for its sharp expertise in visualizing and analysing complex structures as well as dynamic processes. Our field of investigation extends
from the microscopic scale, at the cellular level, to that of the entire organism, allowing
for a thorough understanding of biological phenomena.
Our primary objective is to provide researchers with privileged access to state-of-the-art optical equipment, enabling them to successfully address the most complex scientific challenges. In parallel, our team actively engages in the development and implementation of innovative imaging methods to meet the evolving needs of the scientific community.
Open to all research institutes as well as private industries, our platform serves as
a privileged exchange and collaboration hub conducive to the emergence of new ideas
and the realization of ambitious projects in the fields of biology and medicine.
LOCALISATION
Cell Imaging platform
Institut Imagine, 24 bd du Montparnasse, 75015 Paris
Tel: 01 42 75 43 91
Mail: imagerie.sfr.necker@inserm.fr
The platform is located at the Imagine Institute and has a branch
at the Necker Faculty of Medicine.
STAFF
Meriem GARFA-TRAORÉ
Head - IR INSERM
Béatrice DUREL
IR INSERM
Marine PANZA
IECN Unipersité Paris Cité
Gaël MENASCHÉ, PhD
Scientific Advisor, DR2 INSERM
Elisa HERPIN
IECN Unipersité Paris Cité
EQUIPMENT
MISSION
The main missions of the platform are to:
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advise users in the design and implementation of their experiments,
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assist users in sample preparation for super-resolution microscopy,
expansion microscopy, or tissue clearing, -
provide users with imaging systems best suited to their scientific needs,
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assist and train users on all acquisition systems,
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collaborate with researchers on the use of advanced techniques,
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assist users in interpreting and formatting their results.
STRUCTURAL AND LIVE IMAGING
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4 laser scanning confocal microscopes: 1 Nikon AX, 2 Leica SP8, 1 Leica SP5
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2 spinning disk confocal microscopes: Yokogawa CSU-X1 on Zeiss Axio-observer Z1
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4 wide-field microscopes: 2 Zeiss ApoTome structured illumination microscopes,
1 Nikon TIRF fluorescence microscope, 1 Zeiss Axiovert A1 inverted microscope
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1 high content screening confocal microscope : Opera Phenix (HCS)
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1 fully automated imaging system: Thermo Fisher EVOS M7000
ULTRASTRUCTURE ANALYSIS
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1 STED super-resolution microscope: Leica SP8-gSTED
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1 STORM super-resolution microscope: "home-made" on Nikon Eclipse Ti
THREE-DIMENSIONAL IMAGING
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1 light sheet microscope: Zeiss Lightsheet Z1
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1 micro-computed tomography (microCT): Bruker Skyscan 1272
OTHER EQUIPMENT
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1 fluorescence stereomicroscope: Leica M205 FCA
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1 magnifier: Zeiss Stemi 305
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1 refractometer Mettler Toledo
FEES
On request to meriem.garfa@inserm.fr
PUBLICATIONS
2023
A.Claude-Taupin, P.Isnard, A.Bagattin, N.Kuperwasser, F.Roccio, B.Ruscica, N.Goudin, M.Garfa-Traoré, ..., F.Terzi, P.Codogno & N. Dupont
Nature Communications, 2023 December
PMID: 37916190
M.Garfa Traoré, F.Roccio, C.Miceli, G.Ferri, M.Parisot, N.Cagnard, M.Lhomme, N.Dupont, A.Benmerah, S.Saunier, M.Delous
Frontiers in Molecular Biosciences, 2023 October
DOCK11 deficiency in patients with X-linked actinopathy and autoimmunity.
PMID: 36952639
Boussard C, Delage L, Gajardo T, Kauskot A, Batignes M, Goudin N, Stolzenberg MC,
Brunaud C, Panikulam P, Riller Q, Moya-Nilges M, Solarz J, Repérant C, Durel B, ...
Ménager M, Sepulveda FE, Adam F, Rieux-Laucat F.
Blood, 2023 June
A.Tessier, N.Roux, L.Boutaud, E.Lunel, L.Hakkakian, M.Parisot, M.Garfa‐Traoré, ...,
T.Attié‐Bitach and S.Thomas
Acta Neuropathologica Communications, 2023 February
Evaluation of Slowfade Diamond as a buffer for STORM microscopy. PMID: 36874488
Boukhatem H, Durel B, Raimbault M, Laurent A, Olivier N.
Biomed Opt Express, 2023 January.
2022
R.Duclaux-Loras, C.Lebreton, J.Berthelet, F.Charbit-Henrion, O.Nicolle, C.Revenu des Courtils, S.Waich, T.Valovka, A.Khiat, M.Rabant, C.Racine, IC.Guerrera, J.Baptista, M.M Mahe, M.W Hess, B.Durel.... N.Cerf-Bensussan, M.Parlato.
JCI, 2022 May.
H.Garcia, A.Serafin, F.Silbermann, E.Porée, A.Viau, C.Mahaut, K.Billot, E.Birgy,
M.Garfa-Traoré, .... M.Delous, L.Briseño-Roa, S.Saunier.
PNAS, 2022 May
2021
B.Durel, C.Kervrann, G.Bertolin.
Biol Cell, 2021 November
ATG9A protects the plasma membrane from programmed
and incidental permeabilization. PMID: 34257406
Claude-Taupin A, Jia J, Bhujabal Z, Garfa-Traoré M, ..., Reggiori F, Deretic V.
Nat.Cell Biol, 2021 August.
Three-dimensional architecture of nephrons in the normal and cystic kidney.
PMID: 33137337
Blanc T, Goudin N, Zaidan M, Traore MG, Bienaime F, Turinsky L, Garbay S, Nguyen C,
Burtin M, Friedlander G, Terzi F, Pontoglio M.
Kidney Int., 2021 March
D'Aveni M, Notarantonio AB, Agbogan VA, Bertrand A, Fouquet G, Gastineau P,
Garfa-Traoré M, De Carvalho M, Hermine O, Rubio MT, Zavala F.
Front Immunol, 2021 February.