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Fluorescence microscopy plays an essential role in biomedical research, offering perspectives for exploring cellular and tissue mechanisms. Within the SFR Necker, our cellular imaging platform stands out for its sharp expertise in visualizing and analysing complex structures as well as dynamic processes. Our field of investigation extends from the microscopic scale, at the cellular level, to that of the entire organism, allowing for a thorough understanding of biological phenomena.


Our primary objective is to provide researchers with privileged access to state-of-the-art optical equipment, enabling them to successfully address the most complex scientific challenges. In parallel, our team actively engages in the development and implementation of innovative imaging methods to meet the evolving needs of the scientific community.


Open to all research institutes as well as private industries, our platform serves as a privileged exchange and collaboration hub conducive to the emergence of new ideas and the realization of ambitious projects in the fields of biology and medicine.


The platform is located at the Imagine Institute and has a branch at the Necker Faculty of Medicine.


Cell Imaging Platform of the SFR Necker (INSERM US 24)

Imagine Institute, 24 bd du Montparnasse, 75015 Paris

Tel: 01 42 75 43 91




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Meriem Garfa-Traoré, PhD

Manager, IR INSERM

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Gaël Menasché, PhD

Scientific adviser, DR2 INSERM

Béatrice Durel
Marine Panza

M2 Intern - Master Ingénierie de la Santé, Imagerie Cellulaire



The platform has a diverse range of microscopes that cover a wide range of techniques and enable observations at different scales.


For structural and live imaging:

  • 4 laser scanning confocal microscopes: 1 Nikon AX, 2 Leica SP8, 1 Leica SP5

  • 2 spinning disk confocal microscopes: Yokogawa CSU-X1 on Zeiss Axio-observer Z1

  • 4 wide-field microscopes: 2 Zeiss ApoTome structured illumination microscopes, 1 Nikon TIRF fluorescence microscope, 1 Zeiss Axiovert A1 inverted microscope

  • 1 fully automated imaging system: Thermo Fisher EVOS M7000


For ultrastructure analysis:

  • 1 STED super-resolution microscope: Leica SP8-gSTED

  • 1 STORM super-resolution microscope: "home-made" on Nikon Eclipse Ti


For three-dimensional imaging:

  • 1 light sheet microscope: Zeiss Lightsheet Z1

  • 1 micro-computed tomography (microCT): Bruker Skyscan 1272


Other equipment:

  • 1 fluorescence stereomicroscope: Leica M205 FCA

  • 1 magnifier: Zeiss Stemi 305

  • 1 refractometer Mettler Toledo

  • 1 Ibidi Pump System


The main missions of the platform are to:

  • advise users in the design and implementation of their experiments,

  • assist users in sample preparation for super-resolution microscopy, expansion microscopy, or tissue clearing,

  • provide users with imaging systems best suited to their scientific needs,

  • assist and train users on all acquisition systems,

  • collaborate with researchers on the use of advanced techniques,

  • assist users in interpreting and formatting their results.


Image Gallery


Image Gallery



The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells. Nature Communications, 2023 December

A.Claude-Taupin, P.Isnard, A.Bagattin, N.Kuperwasser, F.Roccio, B.Ruscica, N.Goudin, M.Garfa-Traoré, ..., F.Terzi, P.Codogno & N. Dupont


Fluid shear stress triggers cholesterol biosynthesis and uptake in inner medullary collecting duct cells, independently of nephrocystin-1 and nephrocystin-4. Frontiers in Molecular Biosciences, 2023 October

M.Garfa Traoré, F.Roccio, C.Miceli, G.Ferri, M.Parisot, N.Cagnard, M.Lhomme, N.Dupont, A.Benmerah, S.Saunier, M.Delous


DOCK11 deficiency in patients with X-linked actinopathy and autoimmunity. Blood, 2023 June

Boussard C, Delage L, Gajardo T, Kauskot A, Batignes M, Goudin N, Stolzenberg MC, Brunaud C, Panikulam P, Riller Q, Moya-Nilges M, Solarz J, Repérant C, Durel B, ... Ménager M, Sepulveda FE, Adam F, Rieux-Laucat F.


Bi-allelic variations in CRB2, encoding the crumbs cell polarity complex component 2, lead to non-communicating hydrocephalus due to atresia of the aqueduct of sylvius and central canal of the medulla. Acta Neuropathologica Communications, 2023 February

A.Tessier, N.Roux, L.Boutaud, E.Lunel, L.Hakkakian, M.Parisot, M.Garfa‐Traoré, ..., T.Attié‐Bitach and S.Thomas


Evaluation of Slowfade Diamond as a buffer for STORM microscopy. Biomed Opt Express, 2023 January.

Boukhatem H, Durel B, Raimbault M, Laurent A, Olivier N.



UNC45A deficiency causes microvillus inclusion disease-like phenotype by impairing myosin VB-dependent apical trafficking. JCI, 2022 May.

R.Duclaux-Loras, C.Lebreton, J.Berthelet, F.Charbit-Henrion, O.Nicolle, C.Revenu des Courtils, S.Waich, T.Valovka, A.Khiat, M.Rabant, C.Racine, IC.Guerrera, J.Baptista, M.M Mahe, M.W Hess, B.Durel.... N.Cerf-Bensussan, M.Parlato.


Agonists of prostaglandin E2 receptors as potential first in class treatment for nephronophthisis and related ciliopathies. PNAS, 2022 May

H.Garcia, A.Serafin, F.Silbermann, E.Porée, A.Viau, C.Mahaut, K.Billot, E.Birgy, M.Garfa-Traore, .... M.Delous, L.Briseño-Roa, S.Saunier.



Quantitative dSTORM super-resolution microscopy localizes Aurora kinase A/AURKA in the mitochondrial matrix. Biol Cell, 2021 November

B.Durel, C.Kervrann, G.Bertolin.


ATG9A protects the plasma membrane from programmed and incidental permeabilization. Nat.Cell Biol, 2021 August.

Claude-Taupin A, Jia J, Bhujabal Z, Garfa-Traoré M, ..., Reggiori F, Deretic V.


Three-dimensional architecture of nephrons in the normal and cystic kidney. Kidney Int., 2021 March

Blanc T, Goudin N, Zaidan M, Traore MG, Bienaime F, Turinsky L, Garbay S, Nguyen C, Burtin M, Friedlander G, Terzi F, Pontoglio M.


Mobilized Multipotent Hematopoietic Progenitors Promote Expansion and Survival of Allogeneic Tregs and Protect Against Graft Versus Host Disease. Front Immunol, 2021 February.

D'Aveni M, Notarantonio AB, Agbogan VA, Bertrand A, Fouquet G, Gastineau P, Garfa-Traoré M, De Carvalho M, Hermine O, Rubio MT, Zavala F.

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