Presentation

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Fluorescence microscopy is an essential tool in biomedical research.

The SFR Necker Cell Imaging Platform is specialized in visualizing and analyzing structure and dynamic processes, at the cellular and tissue level to the whole organism level.

 

Its mission is to provide researchers with optical instruments and advanced analysis tools, enabling them to respond to their scientific problems.

 

The platform staff also develops new and ever more innovative imaging methods.

 

The platform is open to all research institutes and the private industries.

Localisation 

The platform is located at the Imagine Institute and has a branch in the Faculty of Medicine, University of Paris.

 

Plateforme d'Imagerie Cellulaire de la SFR Necker (UMS 24)

Institut Imagine, 24 bd du Montparnasse, 75015 Paris

Tél: 01 42 75 43 91

imagerie.sfr.necker@inserm.fr

 

Personnel

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Meriem Garfa-Traoré, PhD

Responsable IR Inserm

meriem.garfa@inserm.fr

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Gael Menasché, PhD

Référent scientifique​

gael.menasche@inserm.fr

Béatrice Durel
Sébastien Dupichaud

IE- Université de Paris

sebastien.dupichaud@inserm.fr

 

Equipment

The platform is equipped with several systems to cover a wide range of techniques:

 

• For structural and living imaging:

6 confocal microscopes including two spinning disk (Yokogawa CSU-X1)

3 wide-field microscopes: two structured illuminated microscopes (Zeiss ApoTome) and one Total Internal Reflection Fluorescence Microscope (Nikon)

 

 

• For functional microscopy:

1 microscope for fluorescence lifetime analyzes  and fluorescence correlation spectroscopy (Leica SP8-SMD)

 

• For ultrastructure analysis:

1 STED super-resolution microscope (Leica SP8-STED)

1 STORM super resolution microscope (home made)

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• For in-depth imaging:

1 lightsheet microscope (Zeiss Lightsheet Z1)

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Drosophila larva observed under lightsheet microscope after clearing.

• Other small equipments:

1 loupe

1 fluorescence stereomicroscope

2 epifluorescence microscopes: 1 inverted for cell culture / 1 upright for slides

Fees

The missions of the platform are:

• to advise users in the design and implementation of their experience (sample preparation, choice of fluorochromes, etc.)

• to provide users with the most appropriate imaging systems for their scientific issues

• to help and train users on all acquisition systems

• to collaborate with researchers on the use of advanced techniques, to enable them to go further in understanding biological models

• to assist users in the interpretation and formatting of results

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Publications

1. Quantitative dSTORM super-resolution microscopy localizes Aurora kinase A/AURKA in the mitochondrial matrix. Durel B, Kervrann C, Bertolin G. Biol Cell. 2021 Aug 31. doi: 10.1111/boc.202100021.

2. ATG9A protects plasma membrane from programmed and incidental 1 permeabilization.

Aurore Claude-Taupin, Jingyue Jia, Zambarlal Bhujabal, Meriem Garfa- Traoré, Suresh Kumar, Gustavo Peixoto Duarte da Silva, Ruheena Javed, Yuexi Gu, Lee Allers, Ryan Peters, Fulong Wang, Luciana Jesus da Costa, Sandeep Pallikkuth, Keith A. Lidke, Mario Mauthe, Pauline Verlhac, Yasuo Uchiyama, Michelle Salemi, Brett Phinney, Sharon A. Tooze, Muriel C. Mari, Terje Johansen, Fulvio Reggiori, and Vojo Deretic. Nature Cell Biology, April 2021

 

3. Three-dimensional architecture of nephrons in the normal and cystic kidney.

Thomas Blanc, Nicolas Goudin, Mohamad Zaidan, Meriem Garfa–Traore, Frank Bienaime, Lisa Turinsky, Serge Garbay, Clément Nguyen, Martine Burtin, Gérard Friedlander, Fabiola Terzi, Marco Pontoglio. Kidney International, March 2021

 

4. Mobilized Multipotent Hematopoietic Progenitors Promote Expansion and Survival of Allogeneic Tregs and Protect Against Graft Versus Host Disease -.

Maud D'Aveni, Anne-Béatrice Notarantonio, Viviane A Agbogan, Allan Bertrand, Guillemette Fouquet, Pauline Gastineau, Meriem Garfa-Traoré, Marcelo De Carvalho, Olivier Hermine, Marie-Thérèse Rubio, Flora Zavala. Front Immunol, Feb 2021

 

5. Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids.

Louison Lallemant, Corinne Lebreton, Meriem Garfa–Traoré. Journal of Biological Methods, 2020.

 

6. Light sheet fluorescence microscopy versus confocal microscopy: in quest of a suitable tool to assess drug and nanomedicine penetration into multicellular tumor spheroids.

Lazzari G, Vinciguerra D, Balasso A, Nicolas V, Goudin N, Garfa–Traore M, Fehér A, Dinnyés A, Nicolas J, Couvreur P, Mura S. Eur J Pharm Biopharm. Sept 2019

 

7. Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.

Dupont MA, Humbert C, Huber C, Siour Q, Guerrera IC, Jung V, Christensen A, Pouliet A, Garfa–Traoré M, Nitschké P, Injeyan M, Millar K, Chitayat D, Shannon P, Girisha KM, Shukla A, Mechler C, Lorentzen E, Benmerah A, Cormier–Daire V, Jeanpierre C, Saunier S, Delous M. Hum Mol Genet. Aug 2019

 

8. Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease.

Denis, K., Bris, M. L., Guennec, L. L., Barnier, J.–P., Faure, C., Gouge, A., Bouzinba–Ségard, H., Jamet, A., Euphrasie, D., Durel, B., Barois, N., Pelissier, P., Morand, P. C., Coureuil, M., Lafont, F., Join–Lambert, O., Nassif, X. & Bourdoulous, S. Nature Microbiology, Jun 2019

 

9. Ependymal cilia beating induces an actin network to protect centrioles against shear stress.

Mahuzier A, Shihavuddin A, Fournier C, Lansade P, Faucourt M, Menezes N, Meunier A, Garfa–Traoré M, Carlier MF, Voituriez R, Genovesio A, Spassky N, Delgehyr N. Nat Commun. Jun 2018

 

10. Impaired Transferrin Receptor Palmitoylation and Recycling in Neurodegeneration with Brain Iron Accumulation.

Drecourt A, Babdor J, Dussiot M, Petit F, Goudin N, Garfa–Traoré M, Habarou F, Bole–Feysot C, Nitschké P, Ottolenghi C, Metodiev MD, Serre V, Desguerre I, Boddaert N, Hermine O, Munnich A, Rötig A. Am J Hum Genet. Feb 2018

 

11. Strength of Neisseria meningitidis binding to endothelial cells requires highly-ordered CD147/β 2-adrenoceptor clusters assembled by alpha-actinin-4.

Nawal Maïssa, Valentina Covarelli, Sébastien Janel, Béatrice Durel, Nandi Simpson, Sandra C. Bernard, Liliana Pardo–Lopez, Haniaa Bouzinba–Ségard, Camille Faure, Mark G. H. Scott, Mathieu Coureuil, Philippe C. Morand, Frank Lafont, Xavier Nassif, Stefano Marullo & Sandrine Bourdoulous. Nature Communications Jun 2017