Originally created in 1992 in the Department of Genetics of the Necker Hospital, the CRB-ADN Necker is one of the platforms coordinated by the Fédérative de Recherche Necker (SFR Necker, Inserm US24, CNRS UAR 3633). The CRB-DNA Necker aims to keep human biological samples from patients (as well as members of their families) suffering from rare genetic diseases, either for “safeguard” purposes and subsequently requalified for research projects, or entering directly within the framework of specific research projects. The Necker DNA-CRB is thus unique on the Paris market by its collection of samples from the families of patients monitored. The CRB-ADN Necker is governed by a multi-party agreement established in 2016 between four partners: the Imagine Institute, the AP-HP, the University of Paris and Inserm.
The activities of the CRB-ADN Necker have benefited from recognition as a funded mission (MERRI) since its certification as well as from the support of the Imagine Institute, the AP-HP and the SFR Necker through aid for equipment. and operation (staff / budget line). The CRB-ADN Necker team was thus able to acquire a new DNA extractor and new biological resource management software.
Since its creation, the activity of the CRB-ADN Necker has recorded 219,243 transformations for the initial safeguard requests (which are then essentially requalified in research projects), and 75,590 in the context of specific research projects. The collection is particularly dynamic and is made available to researchers. Indeed, the number of made available of stored samples (outputs) represents more than 50% of recorded preparations.
The CRB-ADN team, in collaboration with the URC, is at the disposal of researchers to help them in the constitution of their collections, participates in national projects "National Infrastructure in Biology and Health" (Biobanks, Rare Disease Cohorts, Institut Hospitalo-Universitaire) and the European Biobanking and Biomolecular Resources Research Infrastructure (BBMRI) program.
The CRB-ADN has been committed to a quality process since 2006 and obtained the NF S96-900 certification in December 2012, renewed in 2015. The CRB has been in its 4th certification cycle since November 2021: NF 96-900 certificate
The commitment of the CRB-ADN in the quality approach is set out in its Quality Policy letter.
Imagine - Institut des maladies génétiques - 1st flor
24 Boulevard du Montparnasse, 75015 Paris
Corinne Antignac, PhD
PUPH University of Paris, APHP, Scientific referent
APHP PRB healthcare manager
The CRB-ADN mainly comprises three rooms with L2 and a large storage room equipped with -80 ° C freezers and gas-phase nitrogen tanks (-190 ° C).
It also has a high throughput DNA extractor (Autopure LS, QIAGEN). The DNA extraction technique is a saline method allowing the use of DNA in various applications such as exome and CGH array.
As part of research projects, the Necker DNA CRB processes the samples it receives as follows:
For blood samples: reception, transformation of samples into derived products in different forms (DNA, leukocyte pellets, peripheral blood lymphocytes, cell lines transformed by EBV).
For skin biopsies: culture of fibroblasts (since 2016).
Secure long-term storage and availability of biological samples, in compliance with national and international regulations.
For a pellet of white blood cells or a DNA extraction, sample is placed on EDTA at room temperature.
For lineage or lymphocytes, sample is placed on citrate or ACD at room temperature.
For the fibroblast culture, the skin biopsy is placed in the transport medium (RPMI HEPES) provided by the CRB.
1. a discharge request form or discharge authorization by email (firstname.lastname@example.org) from the doctor legally responsible for the sample is required.
2. Sample output are by appointment only.
- CLINICAL DIAGNOSTIC samples
Coming from patients and / or their relatives referred by the site's doctors for diagnosis, the coding of acts is done in the GLIMS software from AP-HP.
- RESEARCH samples
Coming from precise and focused research projects, at the request of Inserm headquarters, four tariffs are validated and applied:
"site teams", "Inserm / off site", "academic" and "private".
The CRB sends the depositor, twice a year, a statement of benefits relating to the samples processed during the previous six months. The depositor must initiate the procedure for payment of the costs corresponding to the treatment and / or conservation of said biological resources, according to the principle of a one-stop-shop, provided by Inserm via the SFR Necker.
The pre-project meeting must be organized. It makes it possible to define together the feasibility of the project, the practical modalities of the project, the feasible services and the applicable pricing.