NEWS
2024
NOVEMBER
New Thermo Orbitrap Exploris 240 Mass Spectrometer
in metabolic analyses platform
A metabolic analysis platform has upgraded its capacities with a new, state-of-the-art
LC-MS system. The addition of the Orbitrap Exploris 240 mass spectrometer will significantly enhance the platform's untargeted metabolomics analyses. Its AcquireX™ data acquisition workflow is crucial for these types of studies, enabling the collection of richer,
more informative data.
2023
SEPTEMBER
Necker Proteomics has introduced a new technology dedicated to plasma biomarker research:
Proteograph from Seer.
MARCH
The Proteomic Platform of Necker (PPN) has obtained in March 2023
two important certifications: ISO 9001 and NFX50-900
for “Services and research & development activities in clinical
and translational proteomics”.
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ISO 9001 is an internationally recognized standard that outlines the requirements
for a quality management system. By obtaining this certification, the PPN shows that
it has implemented processes and procedures that ensure consistency in their services.
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NFX50-900 is a French standard that focuses on R&D, as well as the quality of service delivery. This certification demonstrates PPN ability to run high-quality technological research as well as service.
2022
AUGUST
New Sony ID7000 spectral analyser for cytometry platform
The Cytometry Platform has replaced its Sony SP6800 analyser cytometer with the Sony ID7000, the latest development in spectral technology. Its advanced optical design allows the simultaneous analysis of 41 fluorochromes for each cell sample. The SONY ID7000's spectral technology is able to identify the spectral fingerprints of auto-fluorescence signals for analysis and management.
The SONY ID7000 is equipped with a built-in autosampler capable of handling runs
of 24 tubes and 384-well plates and cooling samples to 4°C.
2021
DECEMBER
Label IBiSA
The Necker Proteomic Platform received the label IBiSA as well as IBiSA financial support
to buy a new workstation for sample preparation.
NOVEMBER
One-day seminar of the SFR Necker
First time after the start of the pandemic the one-day seminar of the Structure Fédérative
de Recherche Necker took place on Wednesday, November 17th, 2021 at the Necker Faculty.
The program included presentations from different teams from INEM and Imagine
and the iPSC and LEAT platforms.
Finally a great opportunity for scientific exchanges between members of INEM, Imagine
and core facilities !
FEBRUARY
timsTOF Pro MS (Bruker) - new mass spectrometer
for Proteomics plateform
The Necker Proteomic platform has acquired a timsTOF Pro MS (Bruker) new mass spectrometer (MS) and two liquid chromatography (LC) systems, nanoElute and EvosepONE (cofunded by DIM TG, Imagine, INEM et SFR).
This new instrument presents major innovations: i) separation of ions by ionic mobility (IM) and ii) the fragmentation of all the injected ions (PASEF), allowing to achieve the highest depth of analysis at the highest speed currently possible in proteomics.
Examples of analysis recently optimised on the timsTOF MS include:
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6,000 proteins identified in one shot (90 minutes) in a tissue or cell vs 2000 in 2h on previous intrument
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5000 proteins quantified in 10 spheroids allowing in-depth analysis of low abundance/rare samples (ie 5000 protein identifies in 10 PHH spheroids) vs 1000 in 6h on previous intrument
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380 proteins in undepleted plasma sample (1ul) in 40 min vs 250 in 2h on previous instrument
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1000 proteins in sweat samples (20 ul) in 40 min
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100 samples from a cohort can be analysed in days vs months before
JANUARY
Necker Bio-image Analysis center is created
The Necker Bio-image Analysis was created in January 2021 to meet the biological image analysis needs of the two Institutes and specializes
in the processing and analysis of bio-images.
The work of the cluster is carried out
in scientific collaboration with the users
and the platforms concerned.
2019
Chiara Guerrera: Inserm Innovation 2019 Award
The SFR Necker is proud to announce that Chiara Guerrera, scientific manager of the proteomics platform of the Necker Federative Research Structure,
has received the Inserm Innovation 2019 Award.
Research engineer, Chiara Guerrera has developed a very high level proteomic analysis platform.
The proteomics platform offers state of the art technology and expertise
mostly for clinical and translational studies through its double activity of service and development.
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Recent acquisitions
Cytometry core facility, site Broussais - Spectral analyzer SP6800 SONY
The SP6800 is a very intuitive analyzer cytometer, intended for the autonomous users.
The spectral technology optimizes the sensibility and improves the detection of low signals by collecting the photons from 420nm to 800nm. The spectral technology simplifies the design of multi-panels colors, by eliminating band-pass filters and the conventional matrices of compensation.
The implemented spectral deconvolution makes the simpler and easier analysis by separating the individual spectral imprints of each fluorochrome. We obtain a more complete image of the rare populations. The complexities associated with the work with fluorescent proteins and fluorochromes excited by several lasers are decreased