Presentation

The Genome targeting and editing platform aims to provide experimental and technical expertise to researchers interested in performing targeted or genome-wide screens and the generation of cellular and animal models using the CRISPR-Cas9 system. The platform will work directly to:

 

  • create novel reporter systems

  • optimize relevant strategies

  • design, amplify and package sgRNA libraires

  • assist in reagent and vector generation and creation

  • library sequencing preparation and data analysis

  • incorporate and develop new advances and technologies in the field

PROPOSED SERVICES

We are currently hosting several mouse and human CRISPR inhibition and activation libraries for genome-wide screening:

 

  • Dolomiti_setA (murine inhibition)

  • Dolcetto_setA (human inhibition)

  • Coprano_setA (murine activation)

  • Calabrese_setA (human activation)

  • Human_v2_Inhibition_top5

  • Human_v2-Activation_top5

  • Mouse_v2_Inhibition_top5

 

In addition, the platform has a wide variety of targeting constructs to assist in creating custom cellular and animal models (working directly with the Transgenesis Platform).

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Localisation 

 

Faculty of Medicine, University of Paris, Room 618B
160 rue de Vaugirard
75015 Paris, France

 

tel: +33 (0)1 40 61 53 78

Staff

 
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Nicolas Kuperwasser, PhD
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Marco Pontoglio, PhD

Scientific adviser

marco.pontoglio@inserm.fr

Fees

For year 2022, year of the implementation of the image analysis center, there is no Fees, The fees will be calculated starting from January 2023.